For continuous filiment fibers (such as you have in B-50 or any of the modern fibers)you don't get any increase in srength by plying. What is important (as RightTrailWrongTime said) is that you have equal bundle tension. If one or two strands are tighter then the average, or if one bundle is tighter than the other then you would have unequal stress allowing for easier breakage. The low stretch fibers in fast flight would theoretically be more sensitive to this.
Twisting, plying, and cableing do have a major effect on short staple fibers such as cotton, wool, spun polyester, and flax. Too little twist will allow slippage of the fibers resulting in low break levels, and too much twist can cause a yarn to break at lower levels. Plying does play a major role with these short length fibers as it averages out the unevenness of the strands as well as reducing the liveliness of the yarn.